doi: 10.1016/S0065-2113(10)08002-8, Beneduzi, A., Ambrosini, A., and Passaglia, L. M. P. (2012). We found that the wild type cells colonized the roots significantly better than the ΔcrtB mutant and this behavior is also observed in the 1:1 competition condition (Figure 6C). as well. doi: 10.1111/j.1751-1097.1991.tb01989.x, Kamilova, F., Kravchenko, L. V., Shaposhnikov, A. I., Azarova, T., Makarova, N., and Lugtenberg, B. Plant Microbe Interact. Interestingly, this phenotype was the direct opposite of that shown for P. stewartii (Herrera et al., 2008), suggesting a possible difference in metabolism and preferred growth environment for Pantoea sp. Plant Physiol. Microbiol. Since carotenoids play a role in resistance to reactive oxygen species and, thus, may influence plant association, we constructed a mutant defective in carotenoid production by deleting the crtB gene that encodes for phytoene synthase, the enzyme responsible for the conversion of geranylgeranyl pyrophosphate into phytoene which serves as a precursor for the synthesis of carotenoids (Sandmann and Misawa, 1992). Can. 52, 195–204. doi: 10.1128/Jb.01243–1212. 11, 634–642. There are several possible explanations for the role carotenoids may have in plant association. YR343 clusters (100% of bootstrap replicates) with another Pantoea strain isolated from the Poplar rhizosphere (Pantoea sp. Britton, G. (1989). Norm Van Brocklin, Los An- j geles Rams’ back, will be honored ; as the outstanding pro player. doi: 10.1016/S0378-1119(96)00423-4, Dussault, D., Caillet, S., Le Tien, C., and Lacroix, M. (2008). Briefly, 500 μl of overnight cultures was diluted into 50 ml of M9 minimal medium plus L-Tryptophan (200 μg ml-1 final concentration), and incubated overnight at 28°C. YR343 and GM01 are closely related to P. rwandensis, P. rodasii, P. vagans, and P. eucalypti, all of which were isolated from eucalyptus trees (Coutinho et al., 2002; Brady et al., 2009, 2012). The oxidative burst protects plants against pathogen attack: mechanism and role as an emergency signal for plant bio-defence–a review. Mol. doi: 10.1094/mpmi-12-12-0286-r, PubMed Abstract | CrossRef Full Text | Google Scholar, Alqueres, S. M., Oliveira, J. H., Nogueira, E. M., Guedes, H. V., Oliveira, P. L., Camara, F., et al. For analysis of root colonization, we utilized the YR343-pGFP strain. YR343 was more than 50% identical to those from P. ananatis LMG20103. Pure Appl. Mol. J. Bacteriol. Rice endophyte Pantoea agglomerans YS19 promotes host plant growth and affects allocations of host photosynthates. FIGURE 6. (2001). FEMS Microbiol. Physiol. Microbiol. Lett. 41, 642–650. Crit. YR343 using P. deltoides, T. aestivum (wheat), or A. thaliana as plant hosts. doi: 10.1094/mpmi.2002.15.11.1173, del Amor, F. M., and Cuadra-Crespo, P. (2011). YR343 encodes a single, conserved ipdC gene which encodes indole pyruvate decarboxylase, suggesting that this strain synthesizes IAA using the indole pyruvate pathway (Patten and Glick, 1996). Finally, the loss of carotenoids may affect the ability of Pantoea sp. Microbiol. For example, carotenoids may be needed for survival of Pantoea sp. YR343 was cultured at 28°C in R2A medium (R2A Broth Premix, TEKnova, Inc.) or on R2A agar plates. Biotechnol. Mol. BAC PRO EDPI (Études et Définition de Produits Industriels) La capacité d'accueil en 2nde Pro EDPI au Lycée Rouvière est de 12 élèves. Arch. and some related Klebsiella spp. Functional microdomains in bacterial membranes. Our phylogenetic analyses also indicated that both Pantoea sp. (1974). YR343 were also arranged with an operon structure similar to that of P. ananatis and P. stewartii (Sedkova et al., 2005) (Figure 4A). Phylogenetic analysis was conducted to compare the Pantoea isolate with known species within the Enterobacteriaceae. New functional assignment of the carotenogenic genes crtB and crtE with constructs of these genes from Erwinia species. Krinsky, N. I. Once the shoot tips had rooted, the Poplar plants were transferred to Magenta boxes containing sterile clay soil mixed with 1X Hoagland’s solution (1.63 g Hoagland Modified Basal Salt Mixture per 1 l water, Phytotechnology Laboratories) to provide plant nutrition. doi: 10.1094/MPMI-21-10-1359, Hirayama, O., Nakamura, K., Hamada, S., and Kobayasi, Y. Kloos, K., Mergel, A., Rösch, C., and Bothe, H. (2001). Acta 352, 361–370. All measurements were performed in triplicate and compared to a standard curve generated from IAA (Sigma–Aldrich). Microarray analysis of the gene expression profile induced by the endophytic plant growth-promoting rhizobacteria, Pseudomonas fluorescens FPT9601-T5 in Arabidopsis. Evol. Three plants were tested for each of four conditions, including uninoculated control, wild type only, ΔcrtB only, and a 1:1 mix of wild type and ΔcrtB. Microbiol. (1947). Infect. Spectrochim. Phytopathology 100, 1330–1339. Proc. YR343. 21, 1359–1370.
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