Ravi, V., Chakrabarti, S. K., Makeshkumar, T., Saravanan, R. (2014). Le titulaire de cette licence professionnelle évolue dans un contexte d'ingéniérie collaboratrice. Such a relationship between stele lignification and inability of ARs to develop into SRs was pointed out in sweetpotato in additional studies (Wilson and Lowe, 1973; Belehu et al., 2004). Expression of IbSuSy and AGPb1A was down-regulated 2.2- and 4.0-fold, respectively, as early as 2W, and 8.7- and 24.7-fold, respectively, in 5W ARs. Peru, Cecilia Lafosse. We have previously suggested that major events involved in SR initiation are the reduction of carbon flow towards phenylpropanoid synthesis and its delivery into carbohydrate metabolism and starch biosynthesis (Firon et al., 2013). These time points were chosen to represent (i) the phase in root development during which a developmental decision will be made toward becoming either a storage-root or a lignified non-storage-root (the first 2 weeks after planting; (Villordon et al., 2009) and (ii) the time when SR formation is observed (5W). CTRL, Control (treated with water); GA3, application of 50 ppm gibberellic acid 3 for two weeks; PB, application of 5 ppm paclobutrazol for two weeks. Starch level was determined in ARs (sampled at 2W and 5W after planting) and in SRs (sampled at 5W) following the protocol of (MacRae (1971) with minor modifications. It should be noted that Liebsch et al. Plant Cell. doi: 10.1023/B:PRES.0000011916.0000067519.0000011958, PubMed Abstract | CrossRef Full Text | Google Scholar, Belehu, T., Hammes, P. S., Robbertse, P. J. Recently, a correlation was established in cassava between expression of xylem differentiation- and lignin-biosynthesis-regulators, and fibrous root development (Siebers et al., 2017). In accordance with these developmental effects, GA3 application was found to cause upregulated expression levels of sweetpotato orthologues of vascular development regulators (IbNA075, IbVND7, and IbSND2) and of lignin biosynthesis genes (IbPAL, IbC4H, Ib4CL, IbCCoAOMT, and IbCAD) and downregulation in expression levels of KNOX (IbKN2 and IbKN3) and starch biosynthesis (IbAGPase and IbGBSS) genes. This cascade leads to upregulation of lignin biosynthesis genes, causing high lignification of the AR and development of a non-SR. Adventitious root and LR number was reduced already at 1W after planting by GA3 and not by PB (Figures 2A, C, respectively). Subsequently, samples were cooled down to room temperature and 4 ml of working solution, containing 0.5 ml acetate buffer (2M, pH 4.8), 2.5 ml dH2O, and 1 ml of amyloglucosidase (10 mg/ml), was added to each sample. J'aime bien démonter les pièces, voir comment ça marche. doi: 510.1034/j.1399-3054.2002.1140413.x. (C) Percent (%) root area occupied by xylem vessels (protoxylem, metaxylem and secondary xylem) and xylem fibers. 17.1) . Exogenous gibberellin enhances secondary xylem development and lignification in carrot taproot. Transcript levels of sweetpotato orthologues of the Arabidopsis GA biosynthesis genes IbKO, IbGA20ox, and IbGA3ox and the receptor IbGID1 were highly upregulated by GA3, while transcript levels of genes encoding enzymes of GA deactivation (IbGA2ox1 and IbGA2ox3) were found to be downregulated, matching the induced endogenous GAs levels observed in our study and pointing to the potential existence of a feedforward mechanism. In Arabidopsis, significant metabolic commitment to secondary wall deposition occurs during maturation of xylem vessels, xylary fiber cells, and interfascicular fiber cells in the developing inflorescence stem (Chaffey et al., 2002; Zhong et al., 2006).

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